Our Earlier Lab Reports decomposing the Spike of COVID -19 virus in 6 Hours – 12/2020
Abstract
NCM develops new Plant-Based Treatment Modalities for the treatment of coronavirus disease 2019 (COVID-19)
These plant-based natural mixtures are composed of safe to-use individual ingredients.
Plant based natural mixtures show significant decrease in the amount of coronavirus spike protein, the protein that connects the coronavirus to human cells.
Specifically , in vitro results show a significant decrease in the amount of the Covid-19 ‘spike protein’ within a few hours of incubation.
Background
COVID-19 is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
There is evidence that SARS-CoV-2 utilizes ACE-2 as a cellular entry receptor
ACE-2 is a type I transmembrane metallocarboxypeptidase with homology to ACE, an enzyme longknown to be a key player in the Renin-Angiotensin system (RAS) and a target for the treatment of hypertension.Zhou et al. showed that SARS-CoV-2 could use ACE-2 from humans, Chinese horseshoe bats, civet cats, and pigs to gain entry into ACE-2-expressing HeLa cells.
(Zhou, P., Yang, XL., Wang, XG. et al. A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature 579, 270–273 (2020).
The spike (S) protein of SARS-CoV-2, which plays a key role in the receptor recognition and cell membrane fusion process, is composed of two subunits, S1 and S2.
The S1 subunit contains a receptor-binding domain that recognizes and binds to the host receptor angiotensin-converting enzyme 2 ( ACE-2).
The S2 subunit mediates viral cell membrane fusion by forming a six-helical bundle via the twoheptad repeat domain.
Huang, Y., Yang, C., Xu, Xf. et al. Structural and functional properties of SARS-CoV-2 spike protein: potential antivirus drug development for COVID-19. Acta Pharmacol Sin 41, 1141–1149 (2020)
Results
The natural mixtures developed by NCM revealed significant reduction in the S1 and S2 proteins’ signals , with no reduction in Recombinant SARS Cov -2 Nucleocapsid protein signal
Recombinant SARS-Cov-2 S1 subunit
- The test items were incubated with the Recombinant SARS-Cov-2 S1 subunit protein for 6 hours then run on SDS-page, stained with “instant blue” for the presence of proteins in the gel.
- Following incubation of the protein with Mix B, a 26% reduction in the protein signal was observed.
The test items were incubated with the Recombinant SARS-Cov-2 S2 subunit protein for 6 hours, then run on SDS-page, stained with “instant blue” for the presence of proteins in the gel.
The results showed that:
- Following incubation of the protein with Mix B, a 19% reduction in the protein signal was observed.
- Following incubation of the protein with Mix C, a 27% reduction in the protein signal was observed
- Following incubation of the protein with Mix D, a 47% reduction in the protein signal was observed.
Recombinant SARS Cov -2 Nucleocapsid
Following incubation of the protein with Mix B, Mix C, Mix D, no reduction in the protein signal was observed.
Summary and Conclusions :
In summary, NCM natural mixtures revealed:
Reduction in
- The SARS-Cov-2 S2 S1 subunit ; and
- The SARS-Cov-2 S2 S2 subunit ;
thereby suggesting to lead to a significant reduction in viral loads.
